HIV-1 vector-mediated gene transfer into various subsets of resting human lymphocytes.

Transduction of resting lymphocytes was carried out as described in “Materials and methods.” Relative gene transfer efficiencies of HIV-1 vectors generated in the presence (+) or the absence (Δ) of HIV-1 APs in lymphocyte subsets were determined by analyzing the number of EGFP positive cells 5 days after transduction and by staining with the indicated PE-conjugated mAbs. Data shown are from 1 of 2 independent experiments generating similar results.