Absence of β1 tubulin protein in the absence of NF-E2 in vitro and in vivo.

Immunoblot analysis of (A) purified megakaryocytes from 2 different in vitro cultures and (B) wild-type or p45 NF-E2 mutant blood (left) and spleen (right) samples, using β1 tubulin-specific antiserum, monoclonal antibodies directed against all β tubulin isoforms (pan β in A), or against GAPDH. C indicates a positive control (spleen).