Formation of fibrin - and γ-chain cross-links as analyzed by SDS-PAGE.

First lane of each gel shows a molecular weight marker (102.0 and 81.0 kd) and second lane shows the fibrinogen preparation before incubation with thrombin and FXIII. Following lanes show pairwise incubations of 6.1 μmol/L fibrinogen with 0.5 U/mL human thrombin and 0.1 μmol/L FXIII 34Val (left-hand lanes) or FXIII 34Leu (right-hand lanes) for the times as indicated in the figure.