Fig. 5.
![Fig. 5. Time-course of activation of FXIII by thrombin in the presence of polymerizing fibrin. / The activation of 3.1 μmol/L purified FXIII 34Val (squares) and FXIII 34Leu (circles) by 0.2 U/mL human α-thrombin with the addition of 2.7 μmol/L fibrinogen was analyzed by HPLC. Diamonds represent fibrinopeptide A and triangles fibrinopeptide B. Release of the peptides was expressed as the ratio between the molar quantity of the peptides ([peptide]) and molar quantity of the peptides at full activation ([peptide]f). Results shown are mean values ± SD of 4 experiments. The inset shows a typical chromatogram with 4 defined peaks for fibrinopeptide A, fibrinopeptide B, FXIII 34Val activation peptide, and FXIII 34Leu activation peptide.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/3/10.1182_blood.v96.3.988/5/bloo01557005y.jpeg?Expires=1724236543&Signature=IbbjFeWB2v7dbZ4Hp2fE0wK1OUO6AFUTuje8kO3OS6-oiDUObgYTg78VtTxi3NfhmG29J5bcRETSTLycbAr5qalB9vMBS0OwDBzbrScrNDeEj1EYNebRBIsrZN6w45y7siYXib1EDAbV9Pqu0XkP~6dfl8~qwJb3jlp1LXiR~uFHacKa~tPxBQgGOIvmvK13-cGz6MeKQxSzskXh0RIuhYCJpBJoO6YHNxV7vFi9Ytb-6dicG6C2ZW6CieT6najtHn1~8ifpRTej-NzTZJmsGdVl31U9hyzPst1WGYfBcFP2pcxNrme-hF-zE49vyFwD6XS~lPBzo3KhPcQWp5BCPA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time-course of activation of FXIII by thrombin in the presence of polymerizing fibrin.
The activation of 3.1 μmol/L purified FXIII 34Val (squares) and FXIII 34Leu (circles) by 0.2 U/mL human α-thrombin with the addition of 2.7 μmol/L fibrinogen was analyzed by HPLC. Diamonds represent fibrinopeptide A and triangles fibrinopeptide B. Release of the peptides was expressed as the ratio between the molar quantity of the peptides ([peptide]) and molar quantity of the peptides at full activation ([peptide]f). Results shown are mean values ± SD of 4 experiments. The inset shows a typical chromatogram with 4 defined peaks for fibrinopeptide A, fibrinopeptide B, FXIII 34Val activation peptide, and FXIII 34Leu activation peptide.
