Fig. 4.
![Fig. 4. Activation of FXIII 34Val and FXIII 34Leu by human -thrombin as analyzed by HPLC. / (A) FXIII (4.0 μmol/L) was incubated with increasing doses of thrombin for 1 hour at 37°C. (B) FXIII (4.0 μmol/L) was incubated with 0.5 U/mL thrombin at 37°C for increasing periods of time. Squares represent FXIII 34Val and circles represent FXIII 34Leu. Results shown for each experiment are mean values ± SD of 3 replicates. Release of the activation peptides was expressed as the ratio between the molar quantity of the peptides ([AP]) and molar quantity of the peptides at full activation ([AP]f).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/3/10.1182_blood.v96.3.988/5/bloo01557004x.jpeg?Expires=1724236405&Signature=3OnYwv9bG1jKJmak9YgNL~HK89kwbqMAMAIXUT63Am9S9XGjj9q6Y431~0jkymRzzbJP7F-kkTBFLXcqqdEFtBAtKARxmgjqEkRihJ2L0VwMXSOarS4HPWF17-YgH09by-ej8d2bL5UpqdKnlc3PXmd5PXduleEg8YZBI6hqvsT9bhh2dUn9n4B0CKOgffpOe7yGSsPS-JcWa4VI4EfKxyUT8dWN-Qg1Sf~y5aKcGYwuRpxRcCDN6WbPN9ViRibmSAbfxwRXXF7K2zIBFXtJBAaWEIVWAjM8ICiXeWEPy8VS2S1D6nPX8FtGYWSvxWg4TLk-oZH96HqKtSNN9OYAVw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of FXIII 34Val and FXIII 34Leu by human -thrombin as analyzed by HPLC.
(A) FXIII (4.0 μmol/L) was incubated with increasing doses of thrombin for 1 hour at 37°C. (B) FXIII (4.0 μmol/L) was incubated with 0.5 U/mL thrombin at 37°C for increasing periods of time. Squares represent FXIII 34Val and circles represent FXIII 34Leu. Results shown for each experiment are mean values ± SD of 3 replicates. Release of the activation peptides was expressed as the ratio between the molar quantity of the peptides ([AP]) and molar quantity of the peptides at full activation ([AP]f).
