Proteolysis of purified FXIII by thrombin.

The effects of 1 of hour incubation at 37°C with increasing amounts of human α-thrombin on 4.0 μmol/L FXIII 34Leu (A) and FXIII 34Val (B), and proteolysis of 4.0 μmol/L FXIII 34Leu (C) and FXIII 34Val (D) by 0.4 U/mL thrombin after increasing incubation times were analyzed by reducing SDS-PAGE. Formation of a distinct band for the cleaved A subunit shows activation by thrombin.