The effect of conditioned medium from the RD114/MGirL22Y producer cells on the immunophenotype of purified cord blood CD34⁺, CD38⁻ cells.

FACS profiles are shown for the following: (A) purified CD34⁺, CD38⁻ cells before culture; (B) CD34⁺, CD38⁻ cells after 96 hours of culture in serum-free medium without addition of vector particles; (C) CD34⁺, CD38⁻ cells after 96 hours in medium containing RD114-pseudotyped vector particles; and (D) population of cells shown in panel C analyzed for EGFP expression.