Transduction of human cord blood progenitors with RD114-pseudotyped particles.

Target CD34⁺ cells were cultured in serum-free medium for 24 hours and then transduced on retronectin-coated plates at various MOIs. The open bars depict results obtained with amphotropic vector particles, and the solid bars depict results obtained with RD114-pseudotyped vector particles. The percentages of colonies that grew in the presence of 100 nmol/L trimetrexate (TMTX) were compared with the total number of colonies observed in control cultures. The means and standard deviations for 4 replicate experiments are shown.