The ability of EBF to activate the mb-1 andB29 promoters is conserved between human and mouse.

(A) DNA sequences from the mouse and human mb-1 andB29 promoters. Sequence homology is indicated by − and insertions/deletions by *. The defined EBF-binding sites in the mouse promoters38-40 as well as the potential EBF-binding sites in the human promoters are aligned to a consensus EBF-binding site.36 (B) EMSA obtained by competition for binding by hEBF to an excess of mb-1 promoter EBF site by the addition of increasing amounts of PCR-amplified mb-1 orB29 promoters. The gels have been cut. (C) Diagrams indicating the relative luciferase activity obtained after transient transfections of 250 ng of mb-1 (black bars), B29(white bars), or fos (gray bars) promoter-controlled reporter constructs in the absence or presence of 600 ng of hEBF-encoding expression plasmid. The data were collected from 4 transfections in which the amount of DNA was normalized by the addition of empty expression plasmid (cDNA3). Error bars indicate standard deviation.