Immunoprecipitation analysis of carbimazole-dependent antibodies.

Platelets (A) and endothelial cells (EC) (B) were surface-labeled with biotin and lysed. Immunoprecipitation was performed with serum containing DDAbs or mab. Lane 1: mab Gi18 (anti-PECAM-1), lane 2: serum in the presence of the drug, lane 3: serum without the drug, lane 4: serum in the presence of the drug and soluble r-PECAM-1. Immunoprecipitates were analyzed on a 7.5% SDS-PAGE under nonreducing conditions.