Localization of the amino- and carboxyl-termini of the carboxylase.

Microsomes prepared from 293 cells expressing hGC-FLAG or FLAG-hGC were subjected to proteinase K digestion in the presence (+) or absence (-) of 0.5% CHAPS. Part of the protease digestion sample in the absence of CHAPS was further digested by Endo H. The anti-FLAG M2 mAb was used for Western blot analysis.