Flow cytometric analysis of thymocytes in 3-week-old mAF4 mutant mice.

(A) Distribution of the CD4 and CD8 markers in mutant and control mice. (B) CD3⁺ cells (gate R1) on CD4 and CD8 thymocytes in mutant versus control mice. Histograms are plotted as overlays of the control thymocytes (black line) and AF4 mutant thymocytes (gray line). (C) H-2Kb distribution on CD4 and CD8 cell populations. Gates are H-2Kb low (R1), H-2Kb intermediate (R2), and H-2Kb high (R3). (D) CD69 distribution on CD4 and CD8 thymocytes. Data are displayed as dot plots with log scale. Data are representative of 6 control mice and 6 mAF4−/− mice.