GFP expression is largely maintained during myeloid differentiation from stem cells.

T, myeloid, and B cells were isolated from the bone marrow and spleen of a mouse reconstituted for 26 weeks using positive selection with magnetic beads. The molarity of the Southern bands indicates that at least 5 independent stem cell clones contribute most of the hematopoiesis in the animal (arrows a-e). The purity of column-enriched cells was generally 80% to 95% based on reanalysis of enriched cells. Nearly all donor-derived myeloid cells (monocytes and neutrophils) were GFP⁺ in the long-term reconstituted animal.