Fig. 1.
Competitive repopulation assay using GFP+, Ly-5.2+ LT-HSC, and recipient whole bone marrow cells.
Increasing numbers of transduced (GFP+), donor LT-HSC (Ly-5.2+) were mixed with 2 × 105nucleated bone marrow cells of the recipient type (Ly-5.1+). The recipient cells would statistically contain 20 LT-HSC, only 2 of which would home to bone marrow and contribute to the host-derived hematopoiesis that is seen in each reconstituted animal. Numbers in the upper right quadrant of each FACS plot represent the percentage of donor cells in peripheral blood of the recipient animal.
