SDS-PAGE zymography of brain extracts

. (A) SDS-PAGE zymography of brain extracts. Lane 1 is human tPA, lane 2 is a rat kidney extract as a marker for rat uPA, lanes 3 through 6 are extracts of brain 6 hours after reperfusion, and lanes 7 through 10 are extracts 72 hours after reperfusion. Lanes 3 and 7 are ipsilateral to the infarct of PBS- treated animals, lanes 4 and 8 are contralateral to the infarct. Lanes 5 and 9 are ipsilateral to the infarct in neuroserpin-treated animals and lanes 6 and 10 are contralateral. (B) Quantitative image analysis of PA activity from SDS-PAGE zymography of brain extracts 6 hours following reperfusion. The results represent the average fold increase in either tPA or uPA activity ipsilateral to the stroke relative to normal baseline PA activities contralateral to the stroke. PBS and Ns represent animals treated with either PBS or neuroserpin, respectively, and n ≥ 3 for each condition tested.P ≤ .05 relative to the contralateral activity are shown, and errors represent SEM.