Ectopic expression of A-Myb in WEHI 231 cells leads to maintenance of c-myc mRNA levels on anti-IgM treatment.

Total mRNA was isolated from S3 or pAW9 cells in exponential growth (E) or after treatment with 2 μg/mL anti-IgM for 1, 6, or 8 hours. Samples (20 μg) were subjected to Northern blot analysis for mRNA expression of the A-myb and c-myc genes (bottom and center panels, respectively). Positions of the endogenous (end) and exogenous (ex) A-myb are as indicated. Ethidium bromide staining of the filter (bottom panel) was used to confirm integrity and equal loading of mRNA samples.