Fig. 4.
Fig. 4. A-Myb rescues CH33 lymphoma cells from anti-IgM–mediated apoptosis. / CH33 cells were transiently transfected by electroporation with 5 μg pON407 β-Gal expression vector in the presence or absence of 10 μg pLL1 or pCAD1 A-myb expression vectors, or Bluescript DNA, as control. The 2 μg/mL anti-IgM antibody was added to the medium 6 hours after transfection and cells were incubated for an additional 24 hours. Cellular lysates were subjected to β-Gal assay and absorbance quantitated in arbitrary units (vertical axis). The mean and SD are representative of 2 separate experiments, each carried out in triplicate.

A-Myb rescues CH33 lymphoma cells from anti-IgM–mediated apoptosis.

CH33 cells were transiently transfected by electroporation with 5 μg pON407 β-Gal expression vector in the presence or absence of 10 μg pLL1 or pCAD1 A-myb expression vectors, or Bluescript DNA, as control. The 2 μg/mL anti-IgM antibody was added to the medium 6 hours after transfection and cells were incubated for an additional 24 hours. Cellular lysates were subjected to β-Gal assay and absorbance quantitated in arbitrary units (vertical axis). The mean and SD are representative of 2 separate experiments, each carried out in triplicate.

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