Fig. 2.
Ectopic A-Myb expression in WEHI 231 cell clones.
(A) WEHI 231 cells were transfected with 5 μg of the pSV2Neo and 35 μg of the human A-myb expression vector pLL1 (pAW1, -4, -5, -8, and -9) or the pSV2Neo vector alone (S3 and -5) and stable cell lines were selected by G418 resistance. Total mRNA was isolated from the cells in exponential growth, and samples (20 μg) subjected to Northern blot analysis using radiolabeled human A-mybpLL1 DNA, as probe (top panel). The positions of the transcripts from endogenous murine A-myb gene and the exogenous human A-myb gene are as indicated. As control for equal loading and integrity of the mRNA samples, the gel was stained with ethidium bromide (bottom panel).
