Transduction efficiency of vectors in primary CFU-S.

Whole bone marrow cells were transduced either with the HaMDR1Δ34 vector (top row), or the HaMDR1 vector (bottom row). The cells were then injected into irradiated recipients, and DNA was later prepared from day 14 CFU-S colonies. Southern blot analysis was performed to detect the proviral genome by digesting with EcoRI and probing with a full-length MDR1 cDNA. Each lane represents a sample from an individual colony. The lane marked by an asterisk is from an nontransduced CFU-S colony, which serves as a negative control.