Expression of wild-type protein S and protein S Heerlen.

Quantitative analysis of pulse-chase experiments using transient expression in COS-1 cells. Radiolabeled protein S in the media and cell lysates were immunoprecipitated and electrophoresed on 7.5% SDS-PAGE. The radioactivity of the protein S bands in the dried gels was analyzed with a Phospho Imager. The amount of radioactive protein S present in the cell lysates at the beginning of each individual experiment (0 hours) was assigned a value of 100%. Cell indicates the amount of radioactive protein S in cell lysates (○); medium, amount of radioactive protein S in media (□); and total, the sum of protein S in cell and medium (▵). The means ± SEM of 4 independent experiments are shown.