Evolution of CD45RA expression in CD3/CD28-stimulated UC T cells.

Cell surface expression of CD45RA on purified UC T cells was monitored using a PE-conjugated anti-CD45RA monoclonal antibody following 0, 2, 4, and 6 days of mitogen stimulation with immobilized anti-CD3 and anti-CD28 monoclonal antibodies. The percentage of CD45RA⁺cells and the mean fluorescence intensity (MFI), indicative of the level of expression, are indicated. Results are representative of data obtained with 10 different donors.