Tissue factor and GAPDH message expression after treatment of PBMC with homocysteine or LPS by semiquantitative RT-PCR.

(A) Gel. (B) Results of densitometric analysis of the gel, expressing tissue factor message as a ratio to that of the message for the housekeeping gene, GAPDH. HCY, homocysteine. In the lanes marked buffer, LPS, and HCY, PBMCs were incubated with the respective reagent for 4 hours before RNA was extracted. In the lane marked fresh PBMCs, RNA was extracted immediately after isolation of the monocytes.