Rescue of hematopoiesis in GATA-1.05 mouse by transgenic expression of GATA-2.

(A) Genotyping of GATA-1.05::GATA-2 Tgcompound mutant mice. Three sets of primers were used for the genotyping. Primer sets for the neomycin resistance gene (Neo) and Zfy-1 gene were used for detecting GATA-1.05 allele and sex of the embryos, respectively. Another set of primers was used to detect the GATA-2 transgene. Numbers represent sibling embryos from a single litter. (B-D) Gross appearance of a litter of E13.5 embryos from the mating of GATA-1.05 heterozygous female mouse with GATA-2 Tg(+) male mouse. An embryo withGATA-1.05/Zfy-1(+)/GATA-2 Tg(–) genotype was found dead at E13.5 and showed signs of necrosis (B). In contrast, an embryo ofGATA-1.05(+)/Zfy-1(+)/GATA-2 Tg(+) genotype was apparently healthy (G2R mouse; C), albeit slightly smaller in size, compared with WT male embryo (D).