Gel shift assay with VEGF UTR binding to HAF protein and nuclear proteins of Hep3B cells.

(A) Lane 1, probe alone (30 mer); lane 2, GST-HAF + probe; lanes 3, 4, self competition at 100 and 500 molar excess; lanes 5, 6, lack of competition with EP22 at 100 and 500 molar excess; lanes 7, 8, competition with EP17 at 100 and 500 molar excess; lane 9, GST only. (B) Lane 1, probe alone (30 mer); lane 2, 3, nuclear proteins (5 and 10 μg) + probe; lanes 4, self-competition at 100 fold molar excess using 10 μg nuclear proteins; lanes 5, 6, GST-HAF (2 and 5 μg) + probe. The bottom of the gel contains the VEGF 30 mer probe (VG30). The arrows indicate the specific complex.