ZNF198–FGFR1 expression and tyrosine autophosphorylation.

(A) Verification of Ba/F3 cell ZNF198–FGFR1 expression by Western blot analysis using an anti-FGFR1 antibody. (B) Oligomeric ZNF198–FGFR1 fusion proteins (4ZF/MSCV, 10ZF/MSCV, and ΔZF/MSCV) are tyrosyl phosphorylated, whereas ZNF198–FGFR1 fusions lacking the ZNF198 proline-rich region (4ZFΔPR/MSCV and 10ZFΔPR/MSCV) did not self-associate and were not tyrosyl phosphorylated.