Fig. 5.
Treatment of DCs with MgCl2 completely blocks the ATP-induced permeabilization.
DCs were incubated in the presence of 10 μmol/L ethidium bromide and 5 mmol/L MgCl2 before the application of 5 mmol/L ATP. The histogram represents the fluorescence intensity of control DCs (Ct) incubated with ethidium bromide alone, those treated with ATP in the presence of ethidium bromide (ATP), and those subjected to 5 mmol/L ATP in the presence of ethidium bromide plus 5 mmol/L MgCl2(MgCl2-ATP).
