Extracellular ATP induces permeabilization in DCs.

Phase contrast microscopy (left panels) and the respective fluorescence microscopy (right panels) of DCs incubated for 15 minutes at 37°C with lucifer yellow (0.5 mg/mL) either with (A) or without (B) 5 mmol/L ATP. It is clear that ATP treatment enhanced the entrance of the fluorescence dye into the cells. (Magnification, 320×.)