MCP-1 mRNA transcription in the lung increases postirradiation and is potentiated by the presence of allogeneic T cells as assessed by in situ hybridization.
B10.BR recipient mice were preconditioned with TBI (day −1) and given C57BL/6 BM with 15 × 106 spleen cells (BMS) on the day of BMT (day 0). Lung tissues were harvested on day 3 post-BMT and cryosections hybridized with antisense digoxigenin-labeled riboprobe for MCP-1. Stained tissue (using the ELF-97 alkaline phosphatase substrate) was analyzed on an Olympus BX50 WI microscope under a 20 × objective lens with multiphoton confocal MRC-1024 imaging (tissue excited at 370 nm and emission collected at 515 nm). Light field images (stained by hematoxylin and eosin) were also photographed under a 20 × objective lens. Solid arrow indicates pulmonary arteriole; a, alveolar duct; b, bronchiole. Note the increased staining of vessel endothelium, bronchiolar epithelium, and alveolar cells in the BMS group.
