Fig. 2.
Expression of chemokines in lung parenchyma on day 7 post-allogeneic BMT.
B10.BR recipient mice were preconditioned with TBI (7.5 Gy, day −1) with or without Cy (120 mg/kg per day, days −3, −2) as indicated and given C57BL/6 BM without (BM) or with 15 × 106 spleen cells (BMS) on the day of BMT (day 0). Lung tissue total RNA (obtained after exsanguination and BAL of lungs) on day 7 post-BMT was analyzed by RPA as described in “Materials and methods” using the CK5 template from Pharmingen. One of 3 representative experiments is shown. Densitometry data are shown in Figure 3.
