Frequency of CD4⁻CD8⁻, CD4⁺CD8⁺, CD4⁺, and CD8⁺thymocyte subpopulations.

(A) Late gestation Cx43+/+ (n = 4), Cx43+/− (n = 12), and Cx43−/− (n = 6) embryos. (B) Neonatal Cx43+/+ (n = 6), Cx43+/− (n = 5), and Cx43−/− (n = 3) mice. (C) Young adult Cx43+/+ (n = 4) and Cx43+/− (n = 4) mice. The level of statistical significance for each thymocyte subpopulation between different groups of mice is indicated.