Proteolytic digestion of the wild-type and FM1 mutant CUB 5-8 proteins.

Analysis by reducing SDS–PAGE and silver staining. The left panel shows the degradation of the wild-type CUB 5-8 protein with various concentrations of trypsin in the presence (−Ca⁺⁺) and absence (+Ca⁺⁺) of EDTA. The weak band at the position of the asterisk is not a proteolytic degradation product, as seen in the first lane. The right panel shows the similar effect of trypsin on the FM1 mutant CUB 5-8 protein.