Differential subcellular localization of cytochrome c and AIF during the commitment phase of anti-CD2-induced apoptosis as visualized by confocal immunofluorescence microscopy.

(A) In F3 and F4 cells, the punctate cytosolic distribution pattern of cytochrome c differs from the diffuse staining pattern of AIF throughout the cytosol and the nucleus. (B) A microscopic field displays several F3 cells to show that most of them exhibit a differential distribution pattern for AIF and for cytochrome. (C,D) The presence of BOC-D.fmk or Z-VAD.fmk (50 μmol/L) during apoptotic induction and Percoll fractionation does not prevent the mitochondrial release of cytochrome c and AIF in F5 cells.