Morphologic effects of LSP1 on MI-U937 cells depend on the level of LSP1 expression.

MI-U937⁻ (panels A, B, and C), MI-U937⁺, (panels D, E, and F), and MI-U937⁺⁺⁺⁺(panels G, H, and I) were stained with anti-LSP1 primary antibody with Oregon Green conjugated second antibody and with Texas Red phalloidin for F-actin. Photomicrographs shown are the phase-contrast images (panels A, D, and G), the LSP1 images (panels B, E, and H), and the F-actin images (C, F, and I) of the same cells. Note surface projections with LSP1 and F-actin (arrowhead) in MI-U937⁺⁺⁺⁺ cells, but not MI-U937⁺. The bar is 2 μm.