The effect of IL-4 on protein expression of cPLA2, 5-LO, and LTA4 hydrolase.
(A) Time-course study. After PMNs were incubated with 10 ng/mL of IL-4 for various periods of time, the cell lysates were prepared and applied (20 μg protein) on 8% SDS-PAGE and electro-transferred onto nitrocellulose membrane. Western blot analysis was performed with anti-cPLA2, 5-LO, or LTA4 hydrolase antibodies as described in “Materials and methods.” The densities were measured and normalized by control values that were expressed as 1.0. Results were shown as mean ± SEM (n = 4). *P < .05 versus control. (B) Dose-response study. After PMNs were incubated with various concentrations of IL-4 for 6 hours, the cell lysates were applied on SDS-PAGE. Results are shown as mean ± SEM (n = 4). *P < .05 versus control.
