Northern blotting analysis.

The TIS11b expression was examined in leukemia cell lines and in bone marrow cells from patients with t(8;21) AML-M2 and non-t(8;21) AML-M2 (A) and in normal human tissues (B). The membranes were hybridized with human TIS11b cDNA as a probe and rehybridized with human G3PDH or β-actin cDNA. The expression of TIS11b gene is increased in leukemic cells carrying the t(8;21) translocation.