American Society of Hematology

Fig. 3.

$Fig. 3. Association of p85 with 14-3-3. / (A) One milliliter cytosol or the RIPA-extracted actin-cytoskeletal (actin CSK) fraction derived from resting or thrombin-stimulated platelets (2 × 109/mL) was immunoprecipitated using the antibody to 14-3-3ζ (5 μL) or nonimmune sera (Non I) (5 μL) and immunoblotted using antibodies to the p85 subunit of PI 3-kinase. This figure is representative of 3 similar experiments. (B) One milliliter cytosol or the RIPA-extracted actin cytoskeletal (actin CSK) fraction derived from resting or vWF-stimulated platelets (2 × 109/mL) was immunoprecipitated using antibody to 14-3-3ζ (5 μL) and immunoblotted using antibody to either GP Ib or the p85 subunit of PI 3-kinase. This figure is representative of 3 similar experiments.$

Association of p85 with 14-3-3.

(A) One milliliter cytosol or the RIPA-extracted actin-cytoskeletal (actin CSK) fraction derived from resting or thrombin-stimulated platelets (2 × 10⁹/mL) was immunoprecipitated using the antibody to 14-3-3ζ (5 μL) or nonimmune sera (Non I) (5 μL) and immunoblotted using antibodies to the p85 subunit of PI 3-kinase. This figure is representative of 3 similar experiments. (B) One milliliter cytosol or the RIPA-extracted actin cytoskeletal (actin CSK) fraction derived from resting or vWF-stimulated platelets (2 × 10⁹/mL) was immunoprecipitated using antibody to 14-3-3ζ (5 μL) and immunoblotted using antibody to either GP Ib or the p85 subunit of PI 3-kinase. This figure is representative of 3 similar experiments.

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