The effect of different kininogen forms on the binding of ¹²⁵I-VN to immobilized uPAR.

The binding of ¹²⁵I-VN to immobilized uPAR together with 20 nmol/L uPA and 50 μmol/L ZnCl₂ was studied in the absence or presence of HK (open circles), HKa (filled circles), domain 3 (open squares), domain 5 (filled squares), or domain 6 (open triangles). Specific binding is expressed as percentage of control, which represents the binding of ¹²⁵I-VN to uPAR in the absence of uPA. Data are mean ± SEM (n = 3) of a typical experiment, and similar results were obtained in 3 separate experiments.