The effect of inhibiting the NF-κB and/or p38SAPK pathways on the LPS-induced up-regulation of CD80, CD83, CD86, and HLA-DR.

Day-7 immature MoDCs were pretreated with either nothing, a cell-permeable peptide that inhibits NF-κB nuclear translocation (SN50 peptide; 50 μg/mL), the p38SAPK inhibitor SB203580 (40 μmol/L), or both for 2 hours before the addition of LPS (100 ng/mL) for 24 hours. Control cells were pretreated with a control peptide plus or minus LPS. The cell surface expression of CD80, CD83, CD86, and HLA-DR was then measured using the flow cytometer. Figures obtained are the product of the percentage of cells expressing the various cell surface antigens and the mean cell fluorescence of the whole population of cells under scrutiny. The value obtained for cells exposed to LPS alone has been normalized to 1. Mean ± SEM of 3 to 4 experiments is shown.