Double staining for CD4/EBER and CD8/EBER.

Double staining of the liver from case 1 shows that (A) the CD4⁺ population harbors the EBV, whereas (B) CD8⁺ population is clearly negative (EBER1 RNA ISH, immunoperoxidase stained with DAB chromogen, hematoxylin counterstained, × 600).