Cytokine production by isolated T-cell clones that are not cytolytic, or proliferative, toward JY cells.

Cytokine production assays were performed with 3 W.B.O. T-cell clones (79, 84, and 89), which are noncytolytic and nonproliferative toward JY cells. CTL clone 110 was included as a positive control. Clone 84 is CD4⁺, the others are CD8⁺. Indicated is the production in log picogram cytokine per million cells per 24 hours. Bars indicate the following: T cells in medium (□), in medium plus PMA and ionomycin (), T cells incubated with JY (▪), or autologous EBV-B (▧) stimulator cells. Note that the scale for IFNγ is 2 logs higher that the other 2. Neither the autologous EBV-B cells nor the JY cells produce any of the 3 cytokines tested here.