American Society of Hematology

Fig. 5.

$Fig. 5. Isolation of viable alloantigen specific T cells from a heterogeneous T-cell pool. / Donor (W.B.O.)-derived unfractionated T cells transduced with SIN-(NFAT)6-GFP were incubated overnight with JY cells as stimulators at a ratio of 1:1. T cells expressing GFP were sorted by means of flow cytometry and subsequently cloned at a single cell per well. As controls, we incubated T cells in medium in the presence or absence of PMA and ionomycin. All cells within the life gate are shown. Transduction efficiency was 30%. The percentage of GFP positive cells in the gate used to sort was approximately 0.2% to 0.3%.$

Isolation of viable alloantigen specific T cells from a heterogeneous T-cell pool.

Donor (W.B.O.)-derived unfractionated T cells transduced with SIN-(NFAT)₆-GFP were incubated overnight with JY cells as stimulators at a ratio of 1:1. T cells expressing GFP were sorted by means of flow cytometry and subsequently cloned at a single cell per well. As controls, we incubated T cells in medium in the presence or absence of PMA and ionomycin. All cells within the life gate are shown. Transduction efficiency was 30%. The percentage of GFP positive cells in the gate used to sort was approximately 0.2% to 0.3%.

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