Expression of GFP in JS136 and melanoma-specific T cell clone.
(A) Induction of GFP expression in T-cell clone JS136 transduced with SIN-(NFAT)6-GFP by its specific target JY is inhibited by anti–HLA-DR antibodies. JS136 T cells transduced with the reporter construct SIN-(NFAT)6-GFP were incubated with JY target cells in the presence or absence of anticlass I (W6/32) or class II (anti–HLA-DQ or -DR) antibodies. Indicated is the percentage of GFP positive T cells after an overnight incubation with JY target cells. Non-JY EBV-B targets were used as negative controls and did not induce GFP expression (data not shown). Stimulation of cells with PMA and ionomycin served as a positive control. Shown are representative data from 3 independent experiments. (B) Melanoma cells induce expression of GFP in a Mart1/MelanA specific CD8+ T-cell clone. Melanoma specific T cells transduced with the reporter construct SIN-(NFAT)6-GFP were incubated with autologous melanoma cells or EBV-B cells. Indicated is the percentage of GFP positive cells. Stimulation of cells with PMA and ionomycin served as a positive control. Shown are representative data from 2 independent experiments.
