American Society of Hematology

Fig. 3.

$Fig. 3. SEB concentration-dependent induction of GFP in SIN-(NFAT)6-GFP–transduced unfractionated T-cell cultures. / SIN-(NFAT)6-GFP–transduced T cells were stimulated overnight in the presence of different concentrations of SEB presented by autologous EBV-B cells. As negative controls, T cells were incubated in medium alone or with the autologous EBV-B cells alone. As a positive control, we incubated cells with PMA in combination with ionomycin. Indicated in the figure is the percentage of GFP positive cells. Shown are representative data from 2 independent experiments.$

SEB concentration-dependent induction of GFP in SIN-(NFAT)₆-GFP–transduced unfractionated T-cell cultures.

SIN-(NFAT)₆-GFP–transduced T cells were stimulated overnight in the presence of different concentrations of SEB presented by autologous EBV-B cells. As negative controls, T cells were incubated in medium alone or with the autologous EBV-B cells alone. As a positive control, we incubated cells with PMA in combination with ionomycin. Indicated in the figure is the percentage of GFP positive cells. Shown are representative data from 2 independent experiments.

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