Activity of the ankyrin-1 gene erythroid promoter in erythroid and nonerythroid cell lines in transient transfection assays.

(A) Plasmids containing 5′ flanking DNA of the ankyrin-1 gene upstream of a firefly luciferase reporter gene were transfected into K562, HEL, and SY5Y (human neuroblastoma), or HeLa cells and relative luciferase activity determined as described. The data are means ± SD of at least 6 independent transfection experiments. (B) Promoter/reporter plasmids containing mutations in consensus DNA-protein binding sites of the ankyrin-1 gene promoter, denoted by the “X” and listed to the right, were transiently transfected into K562 cells and relative luciferase reporter gene expression determined as described.