Bone marrow–derived CD34⁺ cells were enriched with the use of a 2-step method.

Purity was analyzed after Minimacs (A, FSC/SSC; C, CD34/SSC; E, isotype control IgG/SSC), obtaining a mean purity of greater than 95%. Only cells with lymphoblastoid characteristics were sorted. Final purity after FACS with a FACSVantage was greater than 99.5% (B, FSC/SSC; D, CD34/SSC).