Treatment of 32D cell with phosphatase inhibitors.

Viability of parental (A) and WT BAD-expressing (B) 32D cells treated with phosphatase inhibitors. Numbers of viable cells from triplicate wells were determined for each treatment at the indicated times after the removal of IL-3 by trypan blue exclusion. Representative of 3 independent experiments. Similar results were obtained in representative samples when apoptosis was evaluated by DNA content analysis in propidium iodide-stained nuclei. C, control; CyA, cyclosporin A; Cant, cantharidin; and Caly, calyculin.