![Fig. 4. Expression of Jagged2 in endothelial cells but not in fibroblasts. / Total cell lysates were resolved by SDS-PAGE, blotted onto membranes, and probed with anti-ICD antibodies and horseradish peroxidase-conjugated second antibodies. (A) Expression of Jagged2 protein in HUVEC. Lane 1, Rab-9/LXSN (negative control; see below); lane 2, Rab-9/LMJSN (positive control; see below); lane 3, HUVEC; lane 4, S17. The position of the approximately 150-kd Jagged2 protein is indicated. (B) Lack of Jagged2 protein expression in marrow stromal fibroblast cell lines. Lane 1, Rab-9/LXSN (negative control; see below); lane 2, Rab-9/LMJSN (positive control; see below); lane 3, stromal cell line STS; lane 4, stromal cell line STD; lane 5, stromal cell line STW; lane 6, stromal cell line STV; lane 7, stromal cell line S17. The position of the approximately 150-kd Jagged2 protein is shown. (C) Northern blot analysis of Jagged2 expression in endothelial cells and stromal fibroblasts. Each lane contained 5 μg total RNA. The blot was hybridized to a 1:1 mixture of full-length h-Jagged2 and mJagged2 probes. Lane 1, EML C1 (positive control); lane 2, HUVEC; lane 3, HUVEC treated with apolipoprotein-a [apo(a)] for 12 hours; lane 4, STS; lane 5, STD; lane 6, STW; lane 7, STV. The position of 28S rRNA is marked.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/3/10.1182_blood.v96.3.950/5/bloo01543004bw.jpeg?Expires=1724247418&Signature=mZ-joNffDC-WYdr4QGa4Oj-smAJASz7dg3i6s3BtZXshrJTHdI8cZdcFYIWkrBydORMRTy2Pf2jdRbk8YsFnEt4yuLrKistTuqPcb93LzrvAOfBfIFVlqQ5tDZOI~6xZVn3aEMG7o8hT9tQgW~luvQNvizpc0zDsEuiW5wCecu3wGWlw24pgrnkizKNnHilytQh9~QdNcGsBJAIb8frLRRML~UtcBhInrrJXXlnlABwyK3jiLmhKmL2n2w46jrJh-yhQCFRZ5pvpRGQstLa3B41biARn00lfaNmWpzIaYxmXFBPAWM24RQdXyv117IfuVuPI2cnA60moxOEQdGbkXA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of Jagged2 in endothelial cells but not in fibroblasts.
Total cell lysates were resolved by SDS-PAGE, blotted onto membranes, and probed with anti-ICD antibodies and horseradish peroxidase-conjugated second antibodies. (A) Expression of Jagged2 protein in HUVEC. Lane 1, Rab-9/LXSN (negative control; see below); lane 2, Rab-9/LMJSN (positive control; see below); lane 3, HUVEC; lane 4, S17. The position of the approximately 150-kd Jagged2 protein is indicated. (B) Lack of Jagged2 protein expression in marrow stromal fibroblast cell lines. Lane 1, Rab-9/LXSN (negative control; see below); lane 2, Rab-9/LMJSN (positive control; see below); lane 3, stromal cell line STS; lane 4, stromal cell line STD; lane 5, stromal cell line STW; lane 6, stromal cell line STV; lane 7, stromal cell line S17. The position of the approximately 150-kd Jagged2 protein is shown. (C) Northern blot analysis of Jagged2 expression in endothelial cells and stromal fibroblasts. Each lane contained 5 μg total RNA. The blot was hybridized to a 1:1 mixture of full-length h-Jagged2 and mJagged2 probes. Lane 1, EML C1 (positive control); lane 2, HUVEC; lane 3, HUVEC treated with apolipoprotein-a [apo(a)] for 12 hours; lane 4, STS; lane 5, STD; lane 6, STW; lane 7, STV. The position of 28S rRNA is marked.
