Fig. 3.
Fig. 3. Modulation of mJagged2 expression during the proliferation and differentiation of HSC in vitro. Five hundred Lin− c-kit+ RhloHolo cells were cultured in liquid media containing SCF, IL-3, IL-6, and GM-CSF. / Cells were harvested sequentially, and RT-PCR of mJagged2 was performed on serial 5-fold dilutions of cell lysates prepared from 1000 cells. (A) Day 0; (B) day 5; (C) day 8; (D) day 11. The result of β-actin RT-PCR on day 11 is shown at the bottom. First and last lanes contain the φχ174/HaeIII markers.

Modulation of mJagged2 expression during the proliferation and differentiation of HSC in vitro. Five hundred Lin− c-kit+ RhloHolo cells were cultured in liquid media containing SCF, IL-3, IL-6, and GM-CSF.

Cells were harvested sequentially, and RT-PCR of mJagged2 was performed on serial 5-fold dilutions of cell lysates prepared from 1000 cells. (A) Day 0; (B) day 5; (C) day 8; (D) day 11. The result of β-actin RT-PCR on day 11 is shown at the bottom. First and last lanes contain the φχ174/HaeIII markers.

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