MEFV expression in normal and FMF primary fibroblast cultures.

Lane 1: Normal skin fibroblasts. Lane 2: FMF skin fibroblasts. Lane 3: Normal peritoneal fibroblasts. Lane 4: FMF peritoneal fibroblasts. Three experiments were performed for each cell type, each with cells from a different passage (normal skin, passages 4, 6, 7; FMF skin, passages 1, 5, 9; normal peritoneum, passages 2, 3, 4; FMF peritoneum, passages 4, 5, 6). A representative experiment is shown in the figure. (A) RT-PCR performed with the use of the normal MEFV primer. (B) RT-PCR performed with the use of the mutant M694V MEFVprimer. Primers were designed from exon 10. (For details, see “Materials and methods” and the legend to Figure 1.) (C) Percentage of C5a inhibition. A₄₉₀ readings for control C5a-induced MPO release were 0.43 to 0.58.