Semiquantitative RT-PCR assay of MEFV expression.

RT-PCR analysis was performed with the use of primers from exons 8 and 10 as described in “Materials and methods.” The size of the amplified MEFV fragments was 420 bp. Lane 1: Normal human neutrophils; undiluted cDNA sample. Lane 2: Neutrophil cDNA diluted 1:100. Lane 3: Neutrophil cDNA diluted 1:1000. Lane 4: Normal peritoneal fibroblasts, fourth passage; undiluted sample. Lane 5: Normal peritoneal fibroblasts induced for 24 hours with 10 ng/mL IL-1β, undiluted sample. Lane 6: IL-1β–induced peritoneal fibroblasts; cDNA diluted 1:10. Lane 7: IL-1β–induced peritoneal fibroblasts; cDNA diluted 1:100. All undiluted samples included the same amount of RNA in the RT reaction.